哺乳动物体细胞核移植技术研究进展

体细胞核移植技术具有十分诱人的应用前景,在农业、物种保护、医疗等领域已显示出优越性。然而核重构等核移植基础理论方面的研究还很薄弱,致使核移植技术还不很完善,克隆动物还存在着甲基化酶异常调节、不正确的印迹基因表达、X染色体异常激活等错误的表观遗传现象,移植成功率较低,在一定程度上限制了它的发展。根据近几年的研究进展,对核移植技术的应用、方法以及存在的问题作一综述 。

Progress on somatic cell nuclear transfer

Somatic cell nuclear transfer offers exciting opportunities in many areas and brings tremendous changes to agriculture,conservation and therapeutics.However,the fundamental research of nuclear transfer including nuclear reprogramming is not fully understood.Some inappropriate epigenetic modifications have been reported in the clone animals,including abnormal regulation of DNA methyltransferase expression,improper expression of imprinted genes,reactivation of the inactive X chromosome.The low efficiency of nuclear transfer hinders the further progress in some degree.we describe some recent advances in applications,methods and main problems limiting efficiency.