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Simulated microgravity alters the metastatic potential of a human lung adenocarcinoma cell line |
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| Authors: | De Chang Huiwen Xu Yinghua Guo Xuege Jiang Yan Liu Kailong Li Chunxiao Pan Ming Yuan Junfeng Wang Tianzhi Li Changting Liu |
| Institution: | 1. Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Beijing, 100853, China 3. Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China 2. Institute for Medical Device Standardization Administration, National Institute for Food and Drug Control, Beijing, 100050, China 4. The State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, 100094, China |
| Abstract: | Simulated microgravity (SM) has been implicated in affecting diverse cellular pathways. Although there is emerging evidence that SM can alter cellular functions, its effect in cancer metastasis has not been addressed. Here, we demonstrate that SM inhibits migration, gelatinolytic activity, and cell proliferation of an A549 human lung adenocarcinoma cell line in vitro. Expression of antigen MKI67 and matrix metalloproteinase-2 (MMP2) was reduced in A549 cells stimulated by clinorotation when compared with the 1×g control condition, while overexpression of each gene improves ability of proliferation and migration, respectively, under SM conditions. These findings suggest that SM reduced the metastatic potential of human lung adenocarcinoma cells by altering the expression of MKI67 and MMP2, thereby inhibiting cell proliferation, migration, and invasion, which may provide some clues to study cancer metastasis in the future. |
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