Closing in on the resting state of the Shaker K(+) channel |
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Authors: | Pathak Medha M Yarov-Yarovoy Vladimir Agarwal Gautam Roux Benoît Barth Patrick Kohout Susy Tombola Francesco Isacoff Ehud Y |
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Institution: | Biophysics Graduate Program, University of California Berkeley, Berkeley, CA 94720, USA. |
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Abstract: | Membrane depolarization causes voltage-gated ion channels to transition from a resting/closed conformation to an activated/open conformation. We used voltage-clamp fluorometry to measure protein motion at specific regions of the Shaker Kv channel. This enabled us to construct new structural models of the resting/closed and activated/open states based on the Kv1.2 crystal structure using the Rosetta-Membrane method and molecular dynamics simulations. Our models account for the measured gating charge displacement and suggest a molecular mechanism of activation in which the primary voltage sensors, S4s, rotate by approximately 180 degrees as they move "outward" by 6-8 A. A subsequent tilting motion of the S4s and the pore domain helices, S5s, of all four subunits induces a concerted movement of the channel's S4-S5 linkers and S6 helices, allowing ion conduction. Our models are compatible with a wide body of data and resolve apparent contradictions that previously led to several distinct models of voltage sensing. |
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