Nitrogen fixation (15N dilution) with soybeans under Thai field conditions |
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Authors: | Kucey R M N Snitwongse P Chaiwanakupt P Wadisirisuk P Siripaibool C Arayangkool T Boonkerd N Rennie R J |
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Institution: | (1) Agriculture Canada Research Station, T1J 4B1 Lethbridge, Alberta, Canada;(2) Nuclear Research Laboratory Bangkhen, Thailand Department of Agriculture, 10900 Bangkok, Thailand;(3) BNF Resource Center, 10900 Bangkok, Thailand;(4) Esso Chemical Canada, T1J 4A9 Lethbridge, Alberta, Canada |
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Abstract: | Controlled environment and field studies were conducted to determine relationships between various measurements of N2 fixation using soybeans and to use these measures to evaluate a number ofBradyrhizobium japonicum strains for effectiveness in N2 fixation in Thai soils.15N dilution measurements of N2 fixation showed levels of fixation ranging from 32 to 161 kg N ha−1 depending on bacterial strain, host cultivar and location. Midseason measures of N2 fixation were correlated with each other, but not related measures taken at maturity. Ranking ofB. japonicum strains based on performance under controlled conditions in N-free media were highly correlated with rankings based on soybean
seed yields and N2 fixation under field conditions. This study showed that inoculation of soybeans with effectiveB. japonicum strains can result in significant increases in yield and uptake of N through fixation. The most effective strains tested
for use in Thai conditions were those isolated from Thai soils; however, effective strains from other locations were also
of benefit. |
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Keywords: | Bradyrhizobium japonicum strain N2 fixation 15N dilution nodule soybean |
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