Production of galactinol from sucrose by plant enzymes |
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| Authors: | Wakiuchi Nariaki Shiomi Ryohei Tamaki Hajime |
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| Affiliation: | The Graduate School of Science and Technology, Kobe University, Nada, Japan. wakiuchi@kobe-u.ac.jp |
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| Abstract: | Galactinol, 1-O-(alpha-D-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. UDP-Glc was prepared with sucrose and UDP using sucrose synthase partially purified from sweet potato roots. Then, the UDP-Glc was converted to UDP-Gal using yeast UDP-Gal 4-epimerase from a commercial source. Finally, galactinol was produced from the UDP-Gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. The product was identified as galactinol by the retention times of HPLC, alpha-galactosidase digestion, and NMR spectrometry. |
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