PCR-based Specific Detection of Ustilaginoidea virens and Ephelis japonica |
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| Authors: | Y-L Zhou K Izumitsu R Sonoda T Nakazaki E Tanaka M Tsuda C Tanaka |
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| Institution: | Authors' addresses: Kyoto University, Graduate School of Agriculture, Laboratory of Environmental Mycoscience, Kyoto 606-8502, Japan;;Chinese Academy of Agricultural Sciences, Institute of Crop Breeding and Cultivation, Key Laboratory of Crop Genetic and Breeding, Beijing 100081, P. R. China;;National Agricultural Research Center, Hokuriku detached office, Jouetsu 943-0193, Japan;;Kyoto University Graduate School of Agriculture, Laboratory of Plant Breeding, Kyoto 606-8502, Japan (correspondence to C. Tanaka. E-mail: ) |
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| Abstract: | A PCR‐based technique for detection of clavicipitaceous pathogens in rice and related grasses was developed. The target pathogens were Ustilaginoidea virens, which causes rice false smut, and Ephelis japonica, which causes rice udbatta disease and black choke in grasses. To design specific primers, a comparison was made on genetic diversity on the rDNA internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene of U. virens, Ephelis japonica, as well as some other clavicipitaceous fungi. Each fungus was successfully detected by using a specific primer set with high sensitivity. Species‐specific primers designed here were capable of detecting these pathogens in plant tissues. The PCR detection was consistent with conventional histological observation. This nested PCR assay was sensitive and reliable for the detection of U. virens and E. japonica, and thus can be a used to study disease cycles and early prediction of false smut and udbatta‐disease incidence in fields. |
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| Keywords: | detection Ephelis japonica ITS specific primer Ustilaginoidea virens |
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