Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain |
| |
Authors: | Oh Baek-Rock Seo Jeong-Woo Heo Sun-Yeon Hong Won-Kyung Luo Lian Hua Joe Min-ho Park Don-Hee Kim Chul Ho |
| |
Institution: | a Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South Korea b Interdisciplinary Program of the Graduate School for Bioenergy & Biomaterials, Chonnam National University, Gwangju 500-757, South Korea c Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeongju, Chonbuk 561-756, South Korea d Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeonbuk 580-185, South Korea |
| |
Abstract: | A mutant strain of Klebsiella pneumoniae, termed GEM167, was obtained by γ irradiation, in which glycerol metabolism was dramatically affected on exposure to γ rays. Levels of metabolites of the glycerol reductive pathway, 1,3-propanediol (1,3-PD) and 3-hydroxypropionic acid (3-HP), were decreased in the GEM167 strain compared to a control strain, whereas the levels of metabolites derived from the oxidative pathway, 2,3-butanediol (2,3-BD), ethanol, lactate, and succinate, were increased. Notably, ethanol production from glycerol was greatly enhanced upon fermentation by the mutant strain, to a maximum production level of 21.5 g/l, with a productivity of 0.93 g/l/h. Ethanol production level was further improved to 25.0 g/l upon overexpression of Zymomonas mobilispdc and adhII genes encoding pyruvate decarboxylase (Pdc) and aldehyde dehydrogenase (Adh), respectively in the mutant strain GEM167. |
| |
Keywords: | Glycerol Ethanol production Fermentation Klebsiella pneumoniae Mutant |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|