QTL mapping for resistance against the European corn borer (Ostrinia nubilalis H.) in early maturing European dent germplasm |
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| Authors: | M. Bohn B. Schulz R. Kreps D. Klein A. E. Melchinger |
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| Affiliation: | (1) Institut of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70593 Stuttgart, Germany e-mail: melchinger@uni-hohenheim.de Tel.: ++49-711-459 2334, Fax: ++49-711-459 2343, DE;(2) KWS Saat AG, Grimsehlstrasse 31, 37555 Einbeck, Germany, Present address: R. Kreps, Societe Des Mais Europeens, Centre de Selection de Chartres, Rue du Bois Musquet, 28300 Champhol, France, DE |
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| Abstract: | The European corn borer (ECB, Ostrinia nubilalis Hübner) is a major pest of maize in Central Europe. We mapped and characterized quantitative trait loci (QTLs) involved in resistance of maize against ECB damage, compared them with QTLs for agronomic traits, and evaluated the usefulness of marker-assisted selection (MAS) for improving ECB resistance in early maturing European maize germplasm. A total 226 F3 families from the cross D06 (resistant) × D408 (susceptible), together with 93 RFLP and two SSR markers were used for the QTL analyses. For each F3 family we measured the length of tunnels produced by larval stalk mining (TL), stalk damage ratings (SDR), and relative grain yield (RGY) in field experiments, with two replications in two environments in 1 year. The agronomic traits comprised grain yield under insecticide protection (GYP) and manual ECB larval infestation (GYI), the date of anthesis (ANT), and the in vitro digestibility of organic matter (IVDOM) of stover. Estimates of genotypic variance (σ2 g) were highly significant for all traits. Six QTLs for TL and five QTLs for SDR were detected, explaining about 50.0% of σ2 g. Most QTLs showed additive gene action for TL and dominance for SDR. No QTL was found for RGY. The number of QTLs detected for the agronomic traits ranged from two for GYI to 12 for ANT, explaining 12.5 to 57.3% of σ2 g, respectively. Only a single QTL was in common between the two resistance traits, as expected from the moderate trait correlation and the moderate proportions of σ2 g explained. Based on these results, MAS for improving ECB resistance can be competitive when cost-effective PCR-based marker systems are applied. However, it remains to be established whether the putative QTL regions for ECB resistance detected in the population D06 × D408 are consistent across other early maturing European maize germplasms. Received: 20 December 1999 / Accepted: 6 June 2000 |
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| Keywords: | Insect resistance Ostrinia nubilalis QTL Maize |
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