Isolation and some properties of mitochondria from yeast Candida lipolytica 695]

Mitochondria is obtained from yeast Candida lipolytica 695 grown in the presence of glucose, lactate or citrate. Yeast mitochondria were shown to be practically indistinguishable from animal tissue mitochondria in [ADP]/[O] values and in their sensitivity to electron transport inhibitors, to inhibitors and uncoupling agents of oxidative phosphorylation. The only exception was more low value of the respiration control under succinate oxidation. Mitochondria from yeast, grown in the presence of lactate or citrate were capable of the reduction of endogenous pyridine nucleotides under succinate oxidation for the expense of the reverse electron transport. No reverse electron transport from succinate to NAD(P) was observed in mitochondria from yeast grown in the presence of glucose, but it was found under oxidation of alpha-glycerophosphate. All three types of yeast mitochondria were not capable of the reverse electron transport coupled with the pyridine nucleotides reduction under lactate oxidation.