The Activity of Xylose Reductase and Xylitol Dehydrogenase in Yeasts |
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| Authors: | Yablochkova E N Bolotnikova O I Mikhailova N P Nemova N N Ginak A I |
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| Institution: | (1) St. Petersburg State Technological Institute (Technical University), Moskovskii pr. 26, St. Petersburg, 198013, Russia;(2) Department of Molecular Biology and Biological and Organic Chemistry, Petrozavodsk State University, pr. Lenina 33, Petrozavodsk, 185640, Russia |
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| Abstract: | The activity and the cofactor specificity of xylose reductase and xylitol dehydrogenase were studied in extracts of yeasts from the genera Candida, Kluyveromyces, Pachysolen, Pichia,and Torulopsis grown under microaerobic conditions. It was found that xylitol dehydrogenase in all of the yeast species studied is specific for NAD+; xylose reductase in the xylitol-producing species C. didensiae, C. intermediae, C. parapsilosis, C. silvanorum, C. tropicalis, Kl. fragilis, Kl. marxianus, P. guillermondii, andT. molishiama is specific for NADPH; and xylose reductase in the ethanol-producing species P. stipitis, C. shehatae, and Pa. tannophilus is specific for both NADPH and NADH. |
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| Keywords: | xylose-assimilating yeasts xylose reductase xylitol dehydrogenase |
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