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A new shuttle vector forBacillus stearothermophilus andEscherichia coli |
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| Authors: | Noriyuki Nakayama Issay Narumi Shinya Nakamoto Hiroshi Kihara |
| Affiliation: | (1) NEC corporation, 4-1-1 Miyazaki, Miyamae-ku, 216 Kawasaki, Kanagawa, Japan;(2) Department of Agricultural Chemistry, Utsunomiya University, 350 Minemachi, 321 Utsunomiya, Tochigi, Japan;(3) Jichi Medical School, School of Nursing, 3311-159 Yakushiji, 329-04 Minamikawachi, Tochigi, Japan |
| Abstract: | Summary A new shuttle vector was constructed by inserting a 3.1 kbp-DNA fragment from thermophilicBacillus sp. plasmid pIH41 intoEscherichia coli plasmid pUC18. The resultant hybrid replicates in bothE. coli andB. stearothermophilus. This vector has ten unique restriction sites within a part oflacZ gene. Insertion of foreign DNA into these sites can be readily detected by a coloration method. |
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