Characterization of a cluster comprising ∼100 odorant receptor genes in mouse |
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Authors: | Shirley Y Xie Paul Feinstein Peter Mombaerts |
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Institution: | (1) The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA, US |
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Abstract: | With ∼1000 genes, the odorant receptor (OR) gene repertoire is the largest gene family in the mouse genome. Here we have
established a 129/Sv BAC contig for mouse OR gene cluster 7 (Olfr7) on Chromosome (Chr) 9. The assembled ∼2-Mb contig consists
of 75 BACs and may contain as many as 100 OR genes, or ∼10% of the mouse repertoire. Facilitated by the lack of introns in
the coding region, we have determined the nucleotide sequence of 37 full-length, 2 partial, and 3 pseudo coding regions. These
42 OR genes and 3 additional OR genes previously mapped to the mouse Olfr7 cluster can be organized into 13 classes based
on OR probe cross-hybridizations with 129/Sv mouse genomic DNA. OR genes belonging to the same class tend to be located next
to each other within the cluster. Comparison of published full-length mouse and rat OR coding sequences with those identified
here shows that the Olfr7 OR genes are highly related to each other, clustering on two major branches of an unrooted phylogenetic
tree. Eight ORs contain an unusual NXC sequon at the amino-terminal extracellular domain that may represent a novel N-linked
glycosylation site. The BAC contig presented here provides the substrate for sequencing of the cluster.
Received: 27 June 2000 / Accepted: 17 August 2000 |
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