A rapid procedure for the isolation of chloroplast DNA fromChlamydomonas using the TL-100 ultracentrifuge |
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| Authors: | David Herrin Terri Worley |
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| Affiliation: | (1) Department of Botany, University of Texas at Austin, 78713 Austin, TX, USA;(2) Present address: Department of Biology, University of California at San Diego, La Jolla, CA, USA |
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| Abstract: | We describe a rapid and efficient procedure for the isolation of chloroplast (and nuclear) DNA from walled cells ofChlamydomonas reinhardtii. Total nucleic acids are prepared and separated by equilibrium centrifugation in CsCl-bisbenzimide gradients using a high-speed table-top ultracentrifuge. Chloroplast DNA sufficient for several restriction analysis is obtained from 1 liter of cells in one to two days. |
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| Keywords: | chloroplast DNA isolation Chlamydomonas |
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