| 无菌小鼠的人工哺乳培育 |
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| 引用本文： | 涂新明,寿克让,高虹,李振杰.无菌小鼠的人工哺乳培育[J].中国实验动物学报,1999,7(1). |
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| 作者姓名： | 涂新明 寿克让 高虹 李振杰 |
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| 作者单位： | 中国医学科学院,中国协和医科大学实验动物研究所,北京 |
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| 摘 要： | 采用无菌子宫摘取术，在隔离器中剥离子宫取仔１６只，生存１５只，在我国首次采用每２４小时插胃管灌胃５～６次的人工哺乳方法，哺乳１５只，离乳６只。粪便、垫料、棉拭子经无菌检查，均无细菌生长。同时对人工乳成分进行了分析比较，比较了人工乳灭菌温度与时间对各营养成分破坏情况，比较了人工乳、豚鼠母乳与小鼠母乳之间的差异
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| 关 键 词： | 人工哺乳 子宫摘取术 剥离子宫 隔离器 无菌检查 |
Production of Germfree Kun Ming(KM) Mice with Artificial Milk by Hand |
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| Tu Xinming, et al.Production of Germfree Kun Ming(KM) Mice with Artificial Milk by Hand[J].Acta Laboratorium Animalis Scientia Sinica,1999,7(1). |
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| Authors: | Tu Xinming |
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| Institution: | Tu Xinming, et al;
(Institute of Laboratory Animal Science, Chinese Academy of;
Medical Sciences, Peking Union Medical College) |
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| Abstract: | ix germfree KM mice were obtained by hysterectomy or caesarian
section, the young were kept in a germfree environment which is called isolator, and were fed
with artificial milk by hand. All newborns were confirmed to be germfree by sterility testing. The
feces, bedding and swab are examined for germfree detection, all test samples are negative,
this result indicate that the germfree animal feeding are successful. Except above, we
analysied nutrition of artificial milk and compared with mother milk of mice. |
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| Keywords: | Hand feeding Caesarian section Tear the uterus Isolator Sterility testing |
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