Instructions for authors |
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| Authors: | Chmielewski Michael Sucajtys Elzbieta Swierczynski Julian Rutkowski Boleslaw Bogusławski Wojciech |
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| Institution: | (1) Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gda sk, Poland;(2) Department of Biochemistry, Transplantology and Internal Medicine, Medical University of Gdask, Poland;(3) Department of Medical Chemistry, Transplantology and Internal Medicine, Medical University of Gdask, Poland |
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| Abstract: | The aim of the present study was to examine hypothesis that the enhanced cholesterologenesis, found in rats with experimental chronic renal failure (CRF) resulted from the increased gene expression of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase – the rate limiting enzyme in the cholesterologenesis pathway, responsible for mevalonate synthesis. Wistar rats were used and experimental CRF was achieved by 5/6 nephrectomy model. We examined: (a) the changes in the rat liver microsomal HMG-CoA reductase activity, (b) the rat liver HMG-CoA reductase mRNA abundance in various times of day. Obtained data indicates that the increased activity of HMG-CoA reductase in the liver of rats with experimental CRF parallel enhanced mRNA level and suggests that enhanced cholesterol biosynthesis, observed in experimental CRF is at least in part due to the increased HMG-CoA reductase gene expression. The results also indicate that the physiological diurnal rhythm of HMG-CoA reductase activity is preserved in the course of experimental CRF. |
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| Keywords: | cholesterol biosynthesis HMG-CoA reductase experimental chronic renal failure hypercholesterolemia |
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