| 精子冷冻保存技术及研究进展 |
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| 引用本文： | 平述煌,杨世华.精子冷冻保存技术及研究进展[J].中国实验动物学杂志,2011(3):67-71. |
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| 作者姓名： | 平述煌 杨世华 |
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| 作者单位： | 昆明理工大学生命科学与技术学院,昆明650224 |
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| 基金项目： | 国家自然科学基金（31071279） |
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| 摘 要： | 精子冷冻是辅助生殖技术的基础,能够有效的保存有价值的基因资源。文章回顾了近些年来国内外精子冷冻保存的重要研究成果,分析了精子的来源、冷冻预处理、冷冻和解冻方法、防冻剂的选择及其加入去除方式的选择等因素对精子冷冻效果的影响。文章还总结了精子冷冻效果的评价方法,展望精子冷冻技术的发展方向和应用前景。
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| 关 键 词： | 精子冷冻 防冻剂 冷冻评估 |
Progress on Sperm Cryopreservation |
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| PING Shu-huang,YANG Shi-hua.Progress on Sperm Cryopreservation[J].Chinese Journal of Laboratory Animal Science,2011(3):67-71. |
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| Authors: | PING Shu-huang YANG Shi-hua |
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| Institution: | (Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650224) |
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| Abstract: | Sperm cryopreservation has revolutionized the field of assisted reproduction,and it can provide an effective way to preserve valuable genetic resources.This review contained information relating to the cryopreservation of animal semen through a summary of significance research results about domestic and foreign sperm cryopreservation in recent years,and the topics discussed that the effect of semen collection,freezing pretreatment,freezing and thawing methodologies,cryoprotectant selection and methods of its addition and removal on sperm cryopreservation.In addition,this review also focus on evaluation criteria for post-thaw spermatozoa,proposing prospect on anticipation about research orientation of sperm cryopreservation and its application. |
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| Keywords: | Sperm cryopreservation Cryoprotectant Freezing-thawing evaluation |
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