胃癌组织中EN01、M2-PK蛋白表达及其临床意义

目的：探讨胃癌组织中烯醇化酶-α（enolase-α，ENOD、肿瘤型丙酮酸激酶（tumor M2pyruvatekinase，M2-PK）的表达、相互关系及临床病理学意义。方法：应用免疫组织化学SP染色方法分别对55例胃癌组织和23例胃良性病变组织切片染色，观察胃癌组织和良性病变组织EN01、M2．PK的表达情况及分析两者临床病理关系。结果：胃癌组织中EN01阳性表达率为67．3％（37／55），明显高于胃良性病变组30．4％（7／23）（P〈0．01），其表达与胃癌分化程度、浸润深度、淋巴结转移及TNM分期均显著相关（均P〈0．05）。M2-PK在胃癌组织中的阳性表达率为78．2％（43／55），明显高于胃良性病变组织39．1％（9／23）（P〈0．01），其表达与胃癌分化程度、浸润深度均显著相关（均P〈0．05）。胃癌组织中EN01与M2-PK的表达呈正相关（r：=0．5729，P〈0．05）。结论：EN01和M2-PK的表达上调与胃癌的发生、发展可能有关，联合检测两种蛋白在肿瘤组织中的表达，对临床判断胃癌的预后有一定的指导意义。

Expressions and Clinical Significances of ENO 1 and M2-PK in Gastric Cancer

Objeetive： To investigate the expression and clinicopathological significance of enolase-α （ENO1） and tumor M2 pyruvate kinase （M2-PK） in gastric cancer. Methods： The expression of ENO1 and M2-PK in 55 paraffin-embedded specimens of gastric cancer and 23 paraffin-embedded specimens of benign gastric tissue was detected by S-P irmnunohistochemistry. The correlation of ENO 1 expression to M2-PK expression, and their correlations to the clinicophathologic features of gastric cancer were analyzed. Results： The positive rate of ENO1 was significantly higher in gastric cancer than in benign gastric tissue （67.3% vs. 30.4%, P〈0.01）, and closely related to differentiation grade （ P〈0.01）, depth of invasion （P〈0.01）, lymph node metastasis （P〈0.05） and TNM staging（ P〈0. 05）.The positive rate of M2-PK was significantly higher in gastric cancer than in benign gastric tissue （78.2%vs. 39.1%, P〈0.01）, and closely related to differentiation grade （ P〈0.05）, depth of invasion （P〈0.05） .The expression of ENO 1 was positively correlated to that of M2-PK （r=0.5729,P〈0.05） . Conclusion： The up-regulation of ENO1 and M2-PK expression may play important roles in the oncogenesis and progression of gastric cancer. Combined detection of the expression of ENO1 and M2-PK in gastric cancer may provide a significant parameter to judge the clinical prognostic of gastric cancer.