血管性痴呆动物模型制作方法的改良

目的对大鼠血管性痴呆双侧颈总动脉永久性结扎模型（permanent bilateral common carotid arteryocclusion,2VO）进行改良,以提高模型动物存活率。方法采取改良造模方法（间隔7d分2次结扎双侧颈总动脉）和传统的2VO方法（同时结扎双侧颈总动脉）建立血管性痴呆模型,观察并比较2种方法大鼠的存活率、学习记忆能力改变及病理形态学变化。结果术后7 d,改良模型组动物存活率（86.7%）明显高于传统模型组（40.0%）（P〈0.05）。术后8 w与12 w,与假手术组比较,两种方法模型组逃避潜伏期均明显延长（P〈0.05）,而改良法与传统法造模组之间相比较无显著性差异。HE染色显示：改良法与传统法造模组的大鼠海马区神经元有相似程度的明显变性、坏死和凋亡。结论 2VO改良造模法是降低大鼠血管性痴呆模型动物死亡率的成功方法,值得进一步研究、推广。

Improvement of Vascular Dementia Model in Rats

Objective To Modify the permanent bilateral common carotid artery（CCA） occlusion（2VO） for vascular dementia（VD） rats model,in order to increase the survival rate of model animals.Methods Vascular dementia was established by the modified 2VO method（ligate unilateral CCA at first,ligate another one 7 days later） and the traditional 2VO method,respectively,and compared the differences of the two kind of methods in survival rates of rats,learning and memory ability and pathological morphology.Results One week after the operations,the survival rate of modified model group animal（86.7%） was obviously higher than traditional model group（40.0%,P〈 0.05）.Morris water maze test results showed that memory abilities in both modified and traditional model group were decreased obviously compared with the sham operation group（P〈 0.05）,and there was no significant difference between modified model group and traditional model group.The neurons in hippocampus in modified and traditional model rats were all shown significant degeneration,necrosis and apoptosis.Conclusion The modified 2VO method is an ideal way to establish rat model of VD.