The glucan-chitin complex in Saccharomyces cerevisiae |
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Authors: | Zdeněk Holan Vladimír Pokorný Karel Beran Antonín Gemperle Zdeněk Tuzar Josef Baldrián |
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Institution: | (1) Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeská 1083, CS-14220 Prague 4, Czechoslovakia;(2) Institute of Physics, Czechoslovak Academy of Sciences, Na Slovance 2, CS-18040 Prague 8, Czechoslovakia;(3) Institute of Macromolecular Chemistry, Czechoslovak Academy of Sciences, Heyrovského nám. 2, CS-16000 Prague 6, Czechoslovakia |
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Abstract: | Scar rings (SR) from scarless cells at the early stages of budding and mature bud scars from Saccharomyces cerevisiae isolated by both chemical and enzymic treatment of cell walls were observed by selected-area electron diffraction (SAED), X-ray diffraction and electron microscopy with simultaneous physico-chemical characterization (including molar mass, intrinsic viscosity and crystallite size) of -chitin and glucan. The SR, composed of glucan with strong 0.608; 0.397 and 0.293 nm X-ray reflections, was formed at the start of budding. The SAED patterns of -chitin both in the adjacent circular zone and in the parts of newly formed primary septum (PS), observed when the development of the PS started, did not differ from those of -chitin in the single mature bud scar. The bud scar consisted of -chitin, glucan and mannan and their content, as well as the crystallite size of chitin, depended on the mode of preparation.Non-Standard Abbreviations ACZ
Adjacent circular zone
- BS
Birth scar
- CFW
Calcofluor white M2R new: Disodium salt of 4,4-bis-(4-anilino-bis-diethyl-amino-S-triazine-2-yl-amino)-2,2-stilbene-disulfonic acid
- EDI
Electron dense istmus
- PS
Primary septum
- SAED
Selected area electron diffraction
- SEM
Scanning electron microscopy
- SR
Scar ring
- TEM
Transmission electron microscopy
- XRD
X-ray diffraction |
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Keywords: | Saccharomyces cerevisiae Bud scar Scar ring Glucan Chitin Mannan Cytology Electron and X-ray diffractions Physico-chemical characterization |
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