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Insect protein digestion improves purity of Steinernema carpocapsae in vitro culture and reduces culture period |
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| Authors: | Cuiling Cao Heng Jian Aiqin Zhao Xia Jiang Qian Liu |
| Affiliation: | 1. Department of Plant Pathology, China Agricultural University, Beijing, 100193, People’s Republic of China 2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China 4. Laboratory of Nematology, Department of Plant Pathology, China Agricultural University, No. 2 Western Yuanmingyuan Road, Haidian District, Beijing, 100193, China 3. School of Natural Resources and Agricultural Sciences, University of Alaska Fairbanks, Fairbanks, AK, USA |
| Abstract: | Insect protein, used for in vitro culture media for entomopathogenic nematode, produces nematodes of high quality. However, the time-consuming culture and poor purity of nematodes hinder the commercial application of insect protein media. We show that hydrolyzed insect protein improves nematode purity in in vitro culture. The results revealed that nematode purity was increased by more than 90 %, and the culture period was reduced by 6 days. Estimated economic efficiency of using hydrolyzed insect protein medium was increased by 44.25 % over that obtained with non-hydrolyzed insect medium. |
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