Genetic diversity among clonal isolates of Karenia brevis as measured with microsatellite markers |
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| Institution: | 1. Department of Biology, Texas A&M University, College Station, TX 77843, USA;2. Center for Biosystematics and Biodiversity, Texas A&M University, College Station, TX 77843, USA;3. Department of Oceanography, Texas A&M University, College Station, TX 77843, USA;9. Departamento de Cirurgia de Cabeca e Pescoco e Otorrinolaringologia, A.C. Camargo Cancer Center, Sao Paulo, SP, BR;99. Grupo de Epidemiologia e Estatistica em Cancer, Centro Internacional de Pesquisa, A.C. Camargo Cancer Center, Sao Paulo, SP, BR;999. Centro de Investigacao Translacional em Oncologia, Institute of Cancer do Estado de Sao Paulo, Sao Paulo, SP, BR;1. Embrapa Forestry, Estrada da Ribeira km. 111, Guaraituba, 83411-000, Colombo, PR, Brazil;2. Soil Science Dept., Universidade Federal do Paraná, Rua dos Funcionários 1540, Setor de Ciências Agrárias, 80035-050, Curitiba, PR, Brazil;1. Department of Chemical Engineering, University of Missouri-Columbia, W2033 Lafferre Hall, Columbia, MO 65211, United States;2. College of Engineering, University of Mustansiriyah, Baghdad, Iraq |
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| Abstract: | Karenia brevis is the major harmful bloom-forming dinoflagellate in the Gulf of Mexico yet little is known about the intraspecific genetic diversity of this species. Here we describe nine new microsatellite markers and, combined with nine previously described microsatellites, use them to genotype 40 cultured isolates of K. brevis. Genetic diversity identified from cultured isolates was compared with the genetic diversity identified from two field samples to assess how well the current cultures represent the field population. Thirty-nine unique haplotypes were identified from 40 cultured isolates of K. brevis using 18 microsatellite markers. Genetic diversity was similar between cultured isolates and the two field samples. The success of 18 microsatellite markers to distinguish individual isolates supports the use of microsatellites as a genetic tool for diagnostic identification of cultured isolates of K. brevis. |
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