Detection of Water in Lipase-Catalyzed Reactions in Reverse Micelles as Studied by Infrared Spectroscopy |
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Authors: | Peter Walde Pier Luigi Luisi |
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Abstract: | The hydrolytic activity of lipolytic enzymes in reverse micelles can be measured continuously with Fourier Transform infrared spectroscopy (FTIR) by following in the region of the OH-stretching band the water consumption during the reaction. This possibility is unique to reverse micellar solutions, because they are optically transparent and because they contain only a limited amount of water. |
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Keywords: | Lipase reverse micelle water infrared |
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