Extracellular nucleases of Bacillus subtilis I. Purification and properties |
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| Affiliation: | 1. Division of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Rangsit Center, Pathumthani, Thailand;2. National Metal and Materials Technology Center, National Science and Technology Development Agency, Pathumthani, Thailand;3. Department of Textile Science, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand;4. Department of Textile Chemistry Technology, Faculty of Industrial Textiles and Fashion Design, Rajamangala University of Technology, Phra Nakhon, Bangkok, Thailand;1. School of Mechanical and Chemical Engineering, The University of Western Australia, Perth, Australia;2. School of Mathematics and Statistics, The University of Western Australia, Perth, Australia;3. School of Mathematics, University of Leeds, Leeds, UK;4. Centre for Offshore Foundation Systems, The University of Western Australia, Perth, Australia;1. Griffith School of Engineering, Griffith University, Australia;2. School of Civil Engineering, The University of Queensland, Australia;3. Salisbury Research Facility, Department of Agriculture and Fisheries, Queensland Government, Australia |
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| Abstract: | Four Ca2+-dependent nucleases, nucleases Bs-10, Bs-IA, Bs-IB and Bs-II, have been purified from a culture fluid of Bacillus subtilis SB19 and their properties investigated. Nucleases Bs-IA, Bs-IB and Bs-II hydrolyze native and denatured DNA and rRNA in the presence of Ca2+. Without the addition of Ca2+, denatured DNA and rRNA are degraded at reduced rates and little degradation of native DNA is found. The activities of these enzymes are inhibited by EDTA and other agents which bind Ca2+. The mode of action of these enzymes on DNA is predominantly exonucleolytic and the major products from DNA are deoxyribonucleoside 3′-monophosphates. However, an endonuclease activity at the level of one-hundredth of the gross nucleolytic activity is detected in the purified preparations of these enzymes. The enzyme nuclease Bs-10 is different from the other three enzymes in that it hydrolyses only denatured DNA and only to a limited extent. |
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