|
|||||
|
|
DNA synthesis during early development of Drosophila melanogaster |
|
| Affiliation: | 1. Department of Genetics and Genome Biology, University of Leicester, Leicester LE1 7RH, UK;2. Electron Microscopy Facility, Core Biotechnology Services, Adrian Building, University of Leicester, University Road, Leicester LE1 7RH, Leicestershire, UK;3. School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK;1. Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri, Kansas City, MO 64110, United States;2. Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66506, United States |
| Abstract: | The synthesis of DNA in D. melanogaster embryos was followed from preblastoderm to gastrula stages using in vitro radiolabelling techniques. Labelled thymidine was incorporated into main-peak nuclear DNA and into a satellite DNA species with a buoyant density of 1·689 g· cm−3 in CsCl. Mitochondrial DNA was not significantly labelled in pre- or postgastrula embryos. Analyses of changes in size and specific activity of the deoxythymidine triphosphate pool in developing embryos were made by high-pressure liquid chromatography and used for the interpretation of temporal changes in patterns of uptake of labelled thymidine into total and acid-insoluble radioactivity. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |