A rapid reliable method for the estimation of pregnanediol in human urine |
| |
| Affiliation: | 1. LEAF - Linking Landscape, Environment, Agriculture and Food Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal;2. Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal;3. Functional Genomics Center Zürich, University of Zurich, Zurich, Switzerland;4. Centre for Interdisciplinary Research in Animal Health (CIISA), Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal;5. Animal Science Department, School of Agrarian and Veterinary Sciences (ECAV), University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal;6. Veterinary and Animal Research Centre (CECAV) and AL4AnimalS, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal;7. Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal;1. Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310018, PR China;2. Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province; Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, China;3. School of Medicine, ShaoXing University, ShaoXing City, Zhejiang Province, China |
| |
| Abstract: | A method is described for the assay of pregnanediol in pregnancy urine as well as in the urine of men, children and non pregnant women. The analytical procedure comprises Amberlite XAD-2 extraction, hot acid hydrolysis, and automated solid injection g.l.c. of the trifluoroacetate derivatives. For low titer urines an additional alumina column Chromatographic step is required. The specificity of the method has been established by g.l.c.-MS; the sensitivity is only limited by the sensitivity of the g.l.c. detector. 0.01 mg pregnanediol/24 h can be determined without difficulty. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
