Rodent stomach sample preparation for nitrogen NIRS analysis |
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| Institution: | 1. Institute of Forest Ecology, Mendel University in Brno, Faculty of Forestry and Wood Technology, Zemedelska 3, 613 00 Brno, Czech Republic;2. Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 612 42 Brno, Czech Republic;3. Ceitec VFU, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 612 42 Brno, Czech Republic;4. Department of Zoology, Fisheries, Hydrobiology and Apiculture, Mendel University in Brno, Faculty of AgriSciences, Zemedelska 1, 613 00 Brno, Czech Republic;5. Central Institute for Supervising and Testing in Agriculture, Hroznova 2, 656 06 Brno, Czech Republic;1. South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Zhuhai Key Laboratory of Marine Bioresource and Environment, School of Marine Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China;2. The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Hong Kong;3. Cetacean Ecology Lab, Cetacea Research Institute, Hong Kong;1. Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal;2. Servei d’Ecopatologia de Fauna Salvatge (SEFaS), Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain;3. Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, Jaén, Spain;4. Agencia de Medio Ambiente y Agua, Isla de la Cartuja, Sevilla, Spain;5. Direcció General de Medi Ambient i Biodiversitat, Depto. de Agricultura, Ramadería, Pesca i Alimentació, Generalitat de Catalunya, Barcelona, Spain;6. Reserva Nacional de Caça dels Ports de Tortosa i Beseit, Roquetes, Tarragona, Spain;7. Institute of Zoology, Zoological Society of London, Regent’s Park, London, United Kingdom;1. Institute of Zoology, National Academy of Sciences of Belarus, Akademicheskaya Street, 27, Minsk 220072, Belarus;2. LIEC—UMR 7360 CNRS, University of Lorraine, Metz, France;3. LISEC EA 2310, Faculté des Sciences du Sport, Strasbourg, France;1. Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, CNRS, Université Lyon 1, Université de Lyon, F-69622, Villeurbanne, France;2. Department of Life and Environmental Sciences, Faculty of Science and Technology, Bournemouth University, Christchurch House, Talbot Campus, Poole, Dorset, BH12 5BB, United Kingdom;3. Centre de Biologie et Gestion des Populations (INRA ⁄ IRD ⁄ Cirad ⁄ Montpellier SupAgro), Campus international de Baillarguet, CS 30016, F-34988, Montferrier-sur-Lez Cedex, France |
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| Abstract: | Near infrared reflectance spectroscopy is a highly promising method for estimating food quality in small mammals. The method used to prepare rodent stomach content samples, however, may influence nitrogenous substance results. In this study, we focus on comparing methods of preparation of samples and finding the optimal preparation method, which means saving time and costs as well as maintenance of reliability and precision of estimation. We examined 1497 stomachs of the following species: Apodemus flavicollis, A. sylvaticus, Clethrionomys glareolus and Microtus arvalis. We used two preparation methods (grinding and abrasion). Where possible, cardiac and pyloric stomach sections were also examined separately. We found no significant difference between these data, hence only financial, time and equipment factors need determine the processing method in future studies. |
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| Keywords: | Diet quality Near-infrared spectroscopy Nitrogenous substances Rodent |
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