sept8a and sept8b mRNA expression in the developing and adult zebrafish |
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| Institution: | 1. University of Würzburg, Biocenter, Division of Electron Microscopy, Würzburg, Germany;2. Research Unit Sensory Biology and Organogenesis, German Research Center for Environmental Health, Helmholtz Zentrum München, Neuherberg, Germany;3. University of Würzburg, Biocenter, Department of Physiological Chemistry, Würzburg, Germany;1. Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, 14977-13111, Iran;2. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, 3619995161, Iran;3. Laboratory of Plant Physiology, Wageningen University, Wageningen, 6700AA, The Netherlands;4. Department of Horticultural Science, Tarbiat Modares University, Terhran, Iran;5. Department of Genetics and Evolution, School of Biological Sciences, The University of Adelaide, Adelaide, Australia;6. Institute of Biotechnology, Shiraz University, Shiraz, Iran;1. Department of Neuroscience, University of Turin Medical School, Turin, Italy;2. Plateau Rosa Laboratories, Breuil-Cervinia, Italy, Zermatt, Switzerland;1. Institut für Mathematik, Emil-Fischer-Strasse 40, 97074 Würzburg, Germany;2. Građevinski fakultet, Sveučilište u Zagrebu, Fra Andrije Kačića Miošića 26, 10000 Zagreb, Croatia;1. Department of Microbiology, Biocentre, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany;2. Division of Electron Microscopy, Biocentre, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany;3. Department of Animal Ecology I, Bayreuth Center of Ecology and Environmental Research (BayCEER), University of Bayreuth, D-95440 Bayreuth, Germany |
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| Abstract: | Septins are highly conserved GTP-binding proteins involved in numerous cellular processes. Despite a growing awareness of their roles in the cell biology, development and signal transmission in nervous systems, comparably little is known about precise septin expression. Here, we use the well-established model organism zebrafish (Danio rerio) to unravel the expression of sept8a and sept8b, with special focus on the CNS. We performed whole mount RNA in situ hybridization on zebrafish 1–4 dpf in combination with serial sectioning of epon-embedded samples as well as on brain sections of adult zebrafish to obtain precise histological mapping of gene expression. Our results show a common expression of both genes at embryonic stages, whereas sept8a is mainly restricted to the gill arches and sept8b to specific brain structures at later stages. Brains of adult zebrafish reveal a large spatial overlap of sept8a and sept8b expression with few regions uniquely expressing sept8a or sept8b. Our results indicate a neuronal expression of both genes, and additionally suggest expression of sept8b in glial cells. Altogether, this study provides a first detailed insight into the expression of sept8a and sept8b in zebrafish and contributes to a more comprehensive understanding of septin biology in vertebrate model systems. |
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| Keywords: | Septin Neuronal development Zebrafish |
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