三七.人参和西洋参细胞悬浮培养的比较研究

用薄层层析对三七、人参和西洋参愈伤组织进行的初步鉴定表明,三种愈伤组织都含有皂甙和主要皂甙成分Rb_1、Rg_1,三七愈伤组织还含有一种抗癌皂甙Rh_1。对愈伤组织的生长,三七低于人参高于西洋参;对愈伤组织中总皂甙含量,三七均高于人参和西洋参。三种植物细胞悬浮培养结果类似于他们的愈伤组织培养,但生长又进一步提高。三七细胞悬浮培养中皂甙产生的时间进程几乎与生长平行,合适的收获期为培养30天。寡糖素不仅增强三七培养细胞的皂甙形成而且促进细胞生长,较合适的浓度为1.25 ppm。通过以上研究,使三七悬浮培养细胞的生长(干重增加178毫克)为最初培养愈伤组织的4倍以上,总皂甙产率高达20.6毫克,为最初培养愈伤组织的8.5倍。

STUDIES ON THE COMPARISON OF CELL SUSPENSION CULTURE BETWEEN PANAX NOTOGINSENG, PANAX GINSENG AND PANAX QUINQUEFOLIUM

Preliminary identification of medicinal compounds in the calluses from P. notoginseng, P. ginseng and P. quinque folium was carried out by TLC. The results demonstrated that the calluses had the potency to synthesize saponin, they also contained the main saponins Rb1 and Rg1. The content of total saponin in the notogineng-callus was higher than those in calluses although the growth of notoginscng-callus was lower than ginseng-callus but higher than quinquefolium-callus. Specifically, the notoginseng-callus contained saponin Rt1 which is an anticanccr. The rerults of cell suspension culture of above three plants was similar to those of callus cultures but the growth confirmed increase. In the cell suspension culture of nol.ogin.-eng, production of saponin proceeded almost in parallel with the cell growth. The optimum time of harvest was culture 30 days. Oligosaccharins as an elicitor not only induced saponin formation but also promoted cell growth, optimum oligosaccharins concentration was about 1.25 ppm. From above studies, the increased dry weight of suspension cell of notoginseng was 178.0 mg and this wa~> up to four times as many as that in original culture callus. The saponin yield in suspension cell reached as high as 20.6 mg which was up to 8.5 times as that ol original culture callus.