Rapid preparation of pure glomeruli and tubular fragments from rabbit kidney : Distribution of alkaline phosphatase |
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| Authors: | P. Mandel J. J. Helwig C. Bollack |
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| Affiliation: | 1. Centre de Neurochimie du CNRS, France;2. Service d''Urologie, Clinique Chirurgicale A, Faculté de Médecine, Strasbourg, France |
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| Abstract: | ![]()
A simple rapid method for separating glomeruli and tubular fragments is described. The alkaline phosphatase activity of the glomeruli based on the tissue proteins, or on the DNA, was about 4%, or 1%, respectively of that of the tubular fraction. Alkaline phosphatase can be used as a chemical marker of the purity of glomerular fractions in studies on the structure, antigenicity, and biochemical function of glomeruli. |
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