PCR-based molecular characterization, phylogenetic analysis and secondary structure of the 28S rDNA of Thaparocleidus wallagonius (Monogenea: Dactylogyridae) ?C the most primitive species of this genus from India |
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Authors: | Chandni Verma Anshu Chaudhary Hridaya Shanker Singh |
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Institution: | Molecular Taxonomy Laboratory, Department of Zoology, University Road, C. C. S. University, Meerut (UP), 250004, India |
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Abstract: | Species of the monogenean genus Thaparocleidus are specific to freshwater siluriform fish. The infection caused by these gill
parasites are a major health problem to fish. But, to focus the control strategies of these parasites, first it is important to establish an
accurate discrimination by molecular methods. In the present study, phylogenetic and structural analysis of 28S region of
ribosomal DNA of T. wallagonius species collected from fish Wallago attu from Meerut (U.P.), India, was carried out. In the first step,
we amplified, sequenced 28S region of ribosomal DNA of T. wallagonius to establish the phylogenetic relationship with other
species of this genus. T. wallagonius found on gill filaments of fish W. attu, is the most primitive parasite of this genus from India,
was unequivocally discriminate from other species of the same genus in this study. A secondary-structure model of the large
subunit rDNA was also predicted using a combined comparative and thermodynamic approach. Molecular morphometric and
phylogenetic relationship of T. wallagonius are discussed in detailed that based on molecular analysis using bioinformatic tools. |
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Keywords: | Monogenea 28S secondary structure India ribosomal DNA |
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