Isolation and identification of an anticancer drug,taxol from <Emphasis Type="Italic">Phyllosticta tabernaemontanae</Emphasis>, a leaf spot fungus of an angiosperm, <Emphasis Type="Italic">Wrightia tinctoria</Emphasis> |
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| Authors: | Rangarajulu Senthil Kumaran Johnpaul Muthumary Byung-Ki Hur |
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| Institution: | (1) Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University, Incheon, 402-751, Republic of Korea;(2) Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, 600-025, India;(3) Present address: W1-3(2111), Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea |
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| Abstract: | Phyllosticta tabernaemontanae, a leaf spot fungus isolated from the diseased leaves of Wrightia tinctoria, showed the production of taxol, an anticancer drug, on modified liquid medium (MID) and potato dextrose broth (PDB) medium
in culture for the first time. The presence of taxol was confirmed by spectroscopic and chromatographic methods of analysis.
The amount of taxol produced by this fungus was quantified using high performance liquid chromatography (HPLC). The maximum
amount of taxol production was recorded in the fungus grown on MID medium (461 μg/L) followed by PDB medium (150 μg/L). The
production rate was increased to 9.2 × 103 fold than that found in the culture broth of earlier reported fungus, Taxomyces andreanae. The results designate that P. tabernaemontanae is an excellent candidate for taxol production. The fungal taxol extracted also showed a strong cytotoxic activity in the
in vitro culture of tested human cancer cells by apoptotic assay. |
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| Keywords: | anticancer drug leaf spot fungus in vitro cytotoxicity test Phyllosticta tabernaemontanae taxol production |
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