Xanthone compounds in shoot cultures of <Emphasis Type="Italic">Gentianella bulgarica</Emphasis> |
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| Authors: | Teodora Janković Branka Vinterhalter Dijana Krstić-Milošević Radomirka Nikolić Dragan Vinterhalter Slobodan Milosavljević |
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| Institution: | (1) Institute for Medicinal Plant Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia;(2) Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia;(3) Faculty of Chemistry, University of Belgrade, Studentski trg 16, PO Box 158, 11000 Belgrade, Serbia |
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| Abstract: | Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l−1 + NAA 0.1 mg l−1. Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents
of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis
revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at
higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were
lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated
by DPPH test. Samples from plants grown in nature showed the highest activity (IC50 = 0.26 mg ml−1), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC50 from 1.6 to 4.4 mg ml−1). |
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