Production of prostaglandins by cells in vitro: radioimmunoassay measurement of the conversion of arachidonic acid to PGE2 and PGF2 alpha |
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| Authors: | F Cohen B M Jaffe |
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| Affiliation: | Departments of Biology and Surgery, Washington University, St. Louis, Missouri 63110 USA |
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| Abstract: | A specific radioimmunoassay has been applied to the measurement of the conversion of arachidonic acid to PGE2 and PGF2α. PGE2 and PGF2α biosynthesis was linearly related to the amount of arachidonic acid added and was significantly inhibited by indomethacin in concentrations as low as 10?10 M. Sonicated Hela, L, and HEp-2 cells synthesized 244.0, 42.3, and 22.6 ng PGE2 per mg of protein, but made substantially less PGF2α. |
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| Keywords: | Address reprint requests to Bernard M. Jaffe Department of Surgery Washington U. 4960 Audubon St. Louis |
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