Synergistic Effects of Bone Mesenchymal Stem Cells and Chondroitinase ABC on Nerve Regeneration After Acellular Nerve Allograft in Rats |
| |
Authors: | Ying Wang Hua Jia Wen-Yuan Li Xiao-Jie Tong Gui-Bo Liu Si-Wen Kang |
| |
Institution: | (1) Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, 110001, China;(2) Departments of Anatomy, Mudanjiang College of Medicine, Mudanjiang, 157011, China;(3) Department of Anatomy, College of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China; |
| |
Abstract: | This study aimed to evaluate whether combination therapy of bone marrow stromal cells (BMSCs) transplantation and chondroitinase
ABC (ChABC) treatment further enhances axonal regeneration and functional recovery after acellular nerve allograft repair
of the sciatic nerve gap in rats. Eight Sprague–Dawley rats were used as nerve donors, and 32 Wistar rats were randomly divided
into four groups: Group I: acellular rat sciatic nerve (ARSN) group; Group II: ChABC treatment; Group III: BMSCs transplantation;
and Group IV: ChABC treatment and BMSCs transplantation. The results showed that compared with ARSN control group, BMSC transplantation
promoted axonal regeneration, the secretion of neural trophic factors NGF, BDNF and axon angiogenesis in nerve graft. ChABC
treatment degraded chondroitin sulfate proteoglycans in ARSN in vitro and in vivo and improved BMSCs survival in ARSN. The
combination therapy caused much better beneficial effects evidenced by increasing sciatic function index, nerve conduction
velocity, restoration rate of tibialis anterior wet muscle weight, and myelinated nerve number, but did not further boost
the therapeutic effects on neurotrophic factor production, axon angiogenesis, and sensory functional recovery by BMSC transplantation.
Taken together, for the first time, we demonstrate the synergistic effects of BMSC transplantation and BMSCs treatment on
peripheral nerve regeneration, and our findings may help establish novel strategies for cell transplantation therapy for peripheral
nerve injury. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|