| 绒毡层及小孢子发育研究的扫描电镜样品制备 |
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| 引用本文： | 陈宝理,金晓萍,张起麟.绒毡层及小孢子发育研究的扫描电镜样品制备[J].植物学通报,1989(2). |
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| 作者姓名： | 陈宝理 金晓萍 张起麟 |
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| 作者单位： | 华中农业大学 武汉
(陈宝理，金晓萍)，武汉大学 武汉(张起麟) |
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| 摘 要： | 锇酸-二甲基亚砜—锇酸冷冻割断法制备的样品,在扫描电镜下,可观察绒毡层及小孢子发育过程,细胞形态保存良好,结构清晰,立体感强,为用扫描电镜研究植物细胞结构提供了一个有应用价值的方法。
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| 关 键 词： | 锇酸-二甲基亚砜-锇酸冷冻割断法 绒毡层 小孢子 周原质团 |
SPECIMEN PREPARATION OF SCANNING ELECTRON MICROSCOPY FOR INVESTIGATION ON DEVELOPMENT OF TAPETUM AND MICROSPORE |
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| Chen Bao-li Jin Xiao-ping.SPECIMEN PREPARATION OF SCANNING ELECTRON MICROSCOPY FOR INVESTIGATION ON DEVELOPMENT OF TAPETUM AND MICROSPORE[J].Chinese Bulletin of Botany,1989(2). |
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| Authors: | Chen Bao-li Jin Xiao-ping |
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| Abstract: | The development of tapetum, microscope and stereoscopic structure of the whole cell were clearly observed inn stamen specimen prepared with osmium-DMSO-osmium freeze cracking method. The results indicated that it is a good method and can be applied on studying cell structure of plant specimen with scanning electron microscopy. |
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| Keywords: | osmium-DMSO-osmium freeze cracking methed tapetum microspore periplasmodium |
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