| 亚硝酸盐对培养心肌细胞周期的影响 |
| |
| 引用本文： | 王光武,赵勇.亚硝酸盐对培养心肌细胞周期的影响[J].细胞生物学杂志,1995,17(3):139-142. |
| |
| 作者姓名： | 王光武 赵勇 |
| |
| 作者单位： | 白求恩医科大学应用基础医学研究所，白求恩医科大学应用基础医学研究所，白求恩医科大学应用基础医学研究所，卫生部放射生物重点实验室 长春 130021，长春 130021，长春 130021 |
| |
| 基金项目： | 国家“八五”攻关课题(85-917-01-01) |
| |
| 摘 要： | 本文用流式细胞仪测定了ＮａＮＯ２对体外培养的Ｗｉｓｔａｒ乳鼠心肌细胞周期的影响。结果表明，１０＾－６ｍｏｌ／Ｌ ＮａＮＯ２引起Ｓ期细胞明显减少（Ｐ〈０．０５）；１０＾－８ｍｏｌ／Ｌ ＮａＮＯ２对其 显著（Ｐ〉０．０５）。应用「３Ｈ」ＴｄＲ掺入法测定了ＮａＮＯ２对心肌细胞增殖的作用。实验发现，１０＾－６ｍｏｌ／Ｌ ＮａＮＯ２明显抑制细胞增殖，而１０＾－９－１０＾７ｍｏｌ／Ｌ的ＮａＮＯ２则促进细胞增殖
|
| 关 键 词： | 亚硝酸盐 药理学 心肌细胞 有丝分裂周期 |
EFFECT OF SODIUM NITRITE ON CELL CYCLE IN CULTURED NEONATAL RAT MYOCARDIOCYTES |
| |
| Wang Guangwu et al..EFFECT OF SODIUM NITRITE ON CELL CYCLE IN CULTURED NEONATAL RAT MYOCARDIOCYTES[J].Chinese Journal of Cell Biology,1995,17(3):139-142. |
| |
| Authors: | Wang Guangwu |
| |
| Abstract: | In our previous work we have demonstrated that sodium nitrite caused acute myocardial necrosis in rats in vivo. The direct effect of sodium nitrite on cell cycle in cultured neonatal rat heart cells and its mechanism were investigated with the following results-. (1) large doses of sodium nitrite (10-6mol/L) could decrease the percentage of S phase of myocytes (P<0.05) (2) The effects of sodium nitrite on 3H]TdR incorporation into DNA of myocytes were; small doses (10-9- 10-7mol/L) was stimulatory, while large dose (10-6mol/L) was inhibitive. (3) Sodium nitrite (10-8-10-6mol/L) could increase the melondialdehyde contents of myocytes. These results suggested that the changes of myocytes induced by sodium nitrite were relative to lipid peroxidation. |
| |
| Keywords: | Cultured myocardiocytes Sodium nitrite Cell cycle |
| 本文献已被 CNKI 维普 等数据库收录！ |
