小鼠造血干细胞增殖和分化的分子生物学证据

本文采用Ｙ染色体特异的性别决定基因（Ｓｒｙ）作为新的细胞遗传标志，通过ＰＣＲ技术来追踪观察造血干细胞的增殖与分化性能。该方法具有简便、灵敏和特异等优点。雌性受体小鼠输注雄鼠骨髓细胞和１３天脾结节（ＣＦＵ－Ｓ１３）细胞后，Ｓｒｙ ＰＣＲ测试受体小鼠的ＣＦＵ－Ｓ结果表明，它们均为供体来源的ＸＹ细胞。用Ｓｒｙ ＰＣＲ骨髓细胞和骨髓中脾结节生成细胞（ＣＰＵ－Ｓ）的长期重建造血能力，结果表明，在存活雌性小鼠

MOLECULAR BIOLOGICAL EVIDENCE ON PROLIFERA- TION AND DIFFERENTIATION OF MURINE HEMATOPOIETIC STEM CELLS

By PCR amplification of Y chromosome specific sex-determining region (Sry) as a new cy-togenetic marker, the characteristics of proliferation and differentiation of hematopoietic stem cells had been studied. The results indicated that this technique had advantages in simplicity, sensitivity, and specificity. When bone marrow cells or 13 day-spleen colony-forming unit (CFU-Sis) derived from male mice were injected into female recipient mice, Sry PCR results showed that all the CFU-S13 and some of cells from bone marrow, spleen, thymus, and lymph node in recipient mice were originated from donor with XY genotype. These data above gave the direct molecular biological evidence that CFU-S could proliferate and differentiate into various hematopoietic cells in vivo during CFU-S transplantation.