Transfer of blood containing primordial germ cells between chicken eggs development of embryonic reproductive tract |
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Authors: | Hiroki Furuta Tomoko Sawada Kaoru Nishikawa Ichiro Yamamoto Tatsuyuki Yoshida Minoru Tanaka |
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Institution: | (1) Department of Animal Science, Nippon Veterinary and Life Science University, Kyounan-cho 1-7-1, Musashino Tokyo, 180-8602, Japan |
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Abstract: | The present study was carried out to investigate development of recipient chicken embryonic reproductive tracts which are
transferred chicken primordial germ cells (PGCs). It is thought that differentiation of PGCs is affected by the gonadal somatic
cells. When female PGCs are transferred to male embryos, it is possible that they differentiate to W-spermatogonia. However,
the relationship development between PGCs and gonads has not been investigated. At stage 12–15 of incubation of fertilized
eggs, donor PGCs, which were taken from the blood vessels of donor embryos, were injected into the blood vessels of recipient
embryos. The gonads were removed from embryos that died after 16 days of incubation and from newly hatched chickens and organs
were examined for morphological and histological features. The survival rate of the treated embryos was 13.6% for homo-sexual
transfer of PGCs (male PGCs to male embryo or female PGCs to female embryo) and 28.9% for hetero-sexual transfer PGCs (male
PGCs to female embryo or female PGCs to male embryo) when determined at 15 days of incubation. The gonads of embryos arising
from homo-sexual transfer appeared to develop normally. In contrast, embryos derived from hetero-sexual transfer of PGCs had
abnormal gonads as assessed by histological observation. These results suggest that hetero-sexual transfer of PGCs may influence
gonadal development early-stage embryos. |
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Keywords: | Chicken Embryo Gonad PGCs Transfer |
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