| 乳腺癌单克隆抗体AF9抗原的特性及分布 |
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| 引用本文： | 蔡桂英,曾文杰.乳腺癌单克隆抗体AF9抗原的特性及分布[J].生物化学与生物物理进展,1994,21(6):521-523. |
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| 作者姓名： | 蔡桂英 曾文杰 |
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| 作者单位： | 华西医科大学生物化学教研室;华西医科大学生物化学教研室 |
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| 基金项目： | 国家自然科学基金资助项目(39070798). |
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| 摘 要： | 对抗人乳腺癌单抗AF9识别的抗原特性及分布进行了研究,结果表明AF9抗原是由糖、脂及蛋白质组成的复合蛋白质,不耐热;AF9识别的抗原决定簇不存在于铁蛋白及癌胚抗原;蛋白质印迹检测表明AF9识别的抗原有4种成分,分子量分别为51 000,56 000,67 000,73 000.免疫组化ABC染色显示该抗原主要存在于乳腺癌细胞的胞浆及胞膜,在部分其它种类肿瘤组织中也可检测到,但在所检正常组织中未见到.AF9抗原可能是新的乳腺肿瘤相关抗原.
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| 关 键 词： | 乳腺癌 肿瘤相关抗原 单克隆抗体 |
| 收稿时间： | 1993/11/10 0:00:00 |
| 修稿时间： | 1994/3/18 0:00:00 |
Characterization and Distribution of the Antigen Recognized by Breast Cacinoma McAb AF9 |
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| Cai Guiying and Zeng Wenjie.Characterization and Distribution of the Antigen Recognized by Breast Cacinoma McAb AF9[J].Progress In Biochemistry and Biophysics,1994,21(6):521-523. |
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| Authors: | Cai Guiying and Zeng Wenjie |
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| Institution: | Department of Biochemistry, West China University of Medical Sciences;Department of Biochemistry, West China University of Medical Sciences |
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| Abstract: | preliminary study was made on the charac-terization and distribution in human tissues ofAF9-recognized antigen. The results demon-strated that the antigen is a complex proteincomposed of carbohydrate,lipid and proteinand susceptible to heating to 100C.The deter-minant of AF9-recognized antigen was not pre-sent in the ferritin and carcinoembryonic anti-gen. Western blotting revealed that AF9-rec-ognized antigen with molecular weights of 51,56.67 and 73 kD.Immunohistochemical stain-ing showed that the antigen mainly existed inthe cytoplasm and on the memibrane of breastcancer cell,and also could be observed in someother tumors.but no staining was detected innormal tissues.The AF9-recognized antigenmay be a new tumor-associated antigen. |
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| Keywords: | breast carcinoma tumor-associated antigen monoclonal antibody |
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