In situ hybridization to metaphase chromosomes in six species ofPhaseolus andVigna using ribosomal DNA as the probe |
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| Authors: | Jianyu Zheng Kohei Irifune Kouji Hirai Masashi Nakata Ryuso Tanaka Hiromichi Morikawa |
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| Institution: | (1) Faculty of Science, Hiroshima University, 724 Higashi-Hiroshima, Japan;(2) Shobara Kakuchi High School, 727 Shobara, Hiroshima, Japan;(3) Botanic Gardens of Toyama, 939 Toyama, Japan;(4) Present address: Department of Biology, Nankai University, 300071 Tianjin, China |
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| Abstract: | In situ hybridization with a biotin-labeled rice ribosomal DNA (rDNA) probe to the somatic metaphase chromosomes of six species ofPhaseolus andVigna (P. angularis, P. calcaratus, P. coccineus, P. vulgaris, V. sesquipedalis andV. sinensis) was done to determine the sites of rDNA. Hybridization signals were present in the terminal and subterminal chromosome regions
of each of the six species. The number of rDNA sites was two inP. angularis andP. calcaratus, four inP. coccineus andP. vulgaris, and six inV. sesquipedalis andV. sinensis. |
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| Keywords: | In situ hybridization Phaseolus Ribosomal DNA Somatic metaphase chromosome Vigna |
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