| 黄曲霉毒素氧化酶/壳聚糖-单壁碳纳米管/聚邻苯二胺修饰电极对杂色曲霉素的检测 |
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| 引用本文： | 陈俊华,李世川,姚冬生,刘大岭.黄曲霉毒素氧化酶/壳聚糖-单壁碳纳米管/聚邻苯二胺修饰电极对杂色曲霉素的检测[J].生物工程学报,2009,25(12):2029-2035. |
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| 作者姓名： | 陈俊华 李世川 姚冬生 刘大岭 |
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| 作者单位： | 暨南大学生命科学技术学院微生物技术研究所,广州,510632 |
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| 基金项目： | 广东省科技攻关项目(No.2005B20401004)资助~~ |
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| 摘 要： | 将黄曲霉毒素氧化酶(AFO)固定在壳聚糖(CS)-单壁碳纳米管(SWCNTs)杂交膜中,组装在聚邻苯二胺(POPD)修饰的金电极(Au)表面,制备了对杂色曲霉素(ST)敏感的生物传感器(AFO/CS-SWCNTs/POPD/Au)。运用原子力显微镜(AFM)、傅立叶变换红外光谱(FT-IR)和交流阻抗技术(EIS)对电极组装过程进行了表征。循环伏安法研究表明,AFO在修饰电极上发生了准可逆的氧化还原反应,是表面控制过程,其式量电位为-0.436V(vs.Ag/AgCl),说明包埋在CS-SWCNTs中的AFO和电极之间发生了直接电子传递。AFO修饰电极对ST具有明显的电催化作用,其表观米氏常数appKM为7.13μmol/L,催化电流与ST浓度在10～310ng/mL范围内呈线性关系,相关系数为0.997,检出限为3ng/mL(S/N=3),响应时间小于10s。组装的生物传感器具有较好的稳定性与重现性,连续检测20ng/mL的ST标准溶液11次,电流值RSD为3.9%;放置一个月后,其电流响应值仍为初始值的85.6%。该方法具有较高的选择性和灵敏度,应用于实际样品检测时,其回收率在87.6%～105.5%之间...
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| 关 键 词： | 黄曲霉毒素氧化酶 杂色曲霉素 单壁碳纳米管 聚邻苯二胺 生物传感器 |
| 收稿时间： | 2009/10/3 0:00:00 |
Detection of sterigmatocystin based on the novel Aflatoxin- Oxidase/chitosan-single-walled carbon nanotubes/poly-o- phenylenediamine modified electrode |
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| Junhua Chen,Shichuan Li,Dongsheng Yao and Daling Liu.Detection of sterigmatocystin based on the novel Aflatoxin- Oxidase/chitosan-single-walled carbon nanotubes/poly-o- phenylenediamine modified electrode[J].Chinese Journal of Biotechnology,2009,25(12):2029-2035. |
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| Authors: | Junhua Chen Shichuan Li Dongsheng Yao and Daling Liu |
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| Institution: | Institute of Microbial Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;Institute of Microbial Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;Institute of Microbial Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;Institute of Microbial Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China |
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| Abstract: | A sensitive electrochemical biosensor based on Aflatoxin-Oxidase (AFO) was developed for detection of sterigmatocystin (ST).The enzyme was immobilized on chitosan-single-walled carbon nanotubes (CS-SWCNTs) hybrid film,which attached to the poly-o-phenylenediamine (POPD)-modified Au electrode.The fabricated procedures of the biosensor were characterized with atomic force microscopy (AFM),fourier transform-infrared spectroscopy (FT-IR),and electrochemical impedance spectroscopy (EIS).The cyclic voltammetric r... |
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| Keywords: | Aflatoxin-Oxidase sterigmatocystin single-walled carbon nanotubes poly-o-phenylenediamine biosensor |
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