Conversion of acid hydrolysate of oil palm empty fruit bunch to L-lactic acid by newly isolated Bacillus coagulans JI12 |
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Authors: | Lidan Ye Mohammad Sufian Bin Hudari Xingding Zhou Dongxu Zhang Zhi Li Jin Chuan Wu |
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Institution: | 1. Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island, Singapore, 627833, Singapore 2. Department of Chemical and Biomolecular Engineering, Faculty of Engineering, National University of Singapore, Blk. E5, 4 Engineering Drive 4, Singapore, 117576, Singapore
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Abstract: | Cost-effective conversion of lignocellulose hydrolysate to optically pure lactic acid is commercially attractive but very challenging. Bacillus coagulans JI12 was isolated from natural environment and used to produce L-lactic acid (optical purity?>?99.5 %) from lignocellulose sugars and acid hydrolysate of oil palm empty fruit bunch (EFB) at 50 °C and pH 6.0 without sterilization of the medium. In fed-batch fermentation with 85 g/L initial xylose and 55 g/L xylose added after 7.5 h, 137.5 g/L lactic acid was produced with a yield of 98 % and a productivity of 4.4 g/L?h. In batch fermentation of a sugar mixture containing 8.5 % xylose, 1 % glucose, and 1 % L-arabinose, the lactic acid yield and productivity reached 98 % and 4.8 g/L?h, respectively. When EFB hydrolysate was used, 59.2 g/L of lactic acid was produced within 9.5 h at a yield of 97 % and a productivity of 6.2 g/L?h, which are the highest among those ever reported from lignocellulose hydrolysates. These results indicate that B. coagulans JI12 is a promising strain for industrial production of L-lactic acid from lignocellulose hydrolysate. |
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