九节龙皂苷诱导胶质瘤SHG-44细胞凋亡的实验观察

目的：探讨九节龙皂苷对胶质瘤SHG-44细胞潜在的治疗作用及其机制。方法：用四基偶唑蓝（MTT）法检测5、7．5、10、12．5、15、20、40、80mg／L九节龙皂苷作用6、12、24、72h对人胶质瘤SHG-44细胞活性的影响和细胞流式术检测SGH-44细胞调亡情况；Hoeehst33258荧光染色法观察细胞形态的变化；琼脂糖凝胶电泳检测SHG-44细胞DNA的完整性。结果：九节龙皂苷明显抑制SHG-44细胞生长活性呈浓度-时间依赖性，并诱导细胞发生明显的凋亡，细胞核发生浓聚边集，DNA呈凋亡特异性“梯状”分布。结论：九节龙皂苷明显抑制SHG-44细胞的生长活性，能引起胶质瘤细胞大量凋亡，具有显著的抗肿瘤作用。

Apoptosis of Glioma SHG-44 Cells Induced by Ardipusilloside and Its Treatment Function

Objective： To study the potential therapeutic effects ofsaponin ardipusilloside on human glioma SHG-44 cells. Methods： Methyl thiazolyl tetrazolium （MTT） assay was performed to measure the effects on the proliferation of SHG-44 cells treated with ardipusilloside at the concentrations of 5,7.5,10,12.5,15,20,40, 80mg/L for 6, 12, 24 and 72 hours. Flowing cell microspectrofluorimetry was use to examine the apotosis of SGH-44 cells. Hoechst 33258 fluorochrome stain assay was used to observe the morphologic change after treatment. DNA agarose gel electrophoresis was used to detect the integrality of DNA. Results： Ardipusilloside significantly inhibited the cell viability of SHG-44 cells in a concentration- and time-dependent manner and induced SHG-44 cells apoptosis characterized by the concentrated cell nucleus and ladder-pattem DNA. Conclusion： Ardipusilloside could effectively suppress the cell viability of glioma SHG-44 cells and induced it apotosis. The anti-cancer bioactivity suggested its prospect of management for human glioma.