| 白花蛇舌草醇提取物对乳腺癌T-47D细胞株生长的抑制作用 |
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| 引用本文： | 张志伟,李瑜,陈雪莲,尹婵,英智,曹建国.白花蛇舌草醇提取物对乳腺癌T-47D细胞株生长的抑制作用[J].生物磁学,2009(13):2436-2439. |
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| 作者姓名： | 张志伟 李瑜 陈雪莲 尹婵 英智 曹建国 |
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| 作者单位： | [1]南华大学医学院肿瘤研究所,湖南衡阳421001 [2]南华大学医学院病理学教研室,湖南衡阳421001 [3]肿瘤细胞与分子病理学湖南省高校重点实验室,湖南衡阳421001 [4],南华大学生命科学院药理学教研室湖南衡阳421001,南华大学生命科学院药理学教研室湖南衡阳421001 |
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| 基金项目： | 湖南省自然科学基金（07JJ6164） |
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| 摘 要： | 目的：观察白花蛇舌草醇提取物对乳腺癌细胞T-47D生长的影响。方法：采用平皿克隆形成实验、软琼脂集落形成实验和BrdUrd（溴脱氧尿苷）掺入实验,观察不同浓度的白花蛇舌草醇提取物（1.0μg/mL,3.0μg/mL,10μg/mL,30μg/mL,100μg/mL）对T-47D细胞锚定依赖性生长和软琼脂集落形成能力及核酸合成的抑制作用。结果：①随着白花蛇舌草醇提取物浓度的升高,对T-47D细胞的生长呈现明显抑制作用;②白花蛇舌草醇提取物可呈浓度依赖性抑制对数生长期T-47D细胞核酸合成。结论：白花蛇舌草醇提取物可能通过影响肿瘤细胞核酸合成而抑制T-47D细胞生长。
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| 关 键 词： | 白花蛇舌草 乳腺癌细胞T-47D 生长抑制 |
Inhibitory Action of Hedyotic Diffusa Alcohol Extractive on Breast Cancer Cell Line T-47D |
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| ZHANG Zhi-wei,LI Yu,CHEN Xue-lian,YIN Chant,YING Zhi,CAO Jian-guo.Inhibitory Action of Hedyotic Diffusa Alcohol Extractive on Breast Cancer Cell Line T-47D[J].Biomagnetism,2009(13):2436-2439. |
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| Authors: | ZHANG Zhi-wei LI Yu CHEN Xue-lian YIN Chant YING Zhi CAO Jian-guo |
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| Institution: | 1 Cancer Research Institute of Medical College, University of Southern China, 421001, Hengyang, China; 2 Department of Pathology of Medical College, University of Southern China, 421001, Hengyang, China; 3 University Key Laboratory of cancer cellular and molecular pathology in Hunan province, 421001, Hengyang, China; 4 Department of Pharmacology of life science College, University of Southern China, 42100 L Hengyang, China ) |
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| Abstract: | Objective： To observe inhibitory action of Hedyotic diffusa alcohol extractive for breast cancer cell line T-47D. Mothotis： The plate clone formation, soft agar colony experiment and BrdUrd （5-bromodeoxyuridine） incorporated experiment were adopted to detect Hedyotic diffusa alcohol extractive of different concentration （1.0μg/mL,3.0μg/mL, 10μg/mL, 30μg/mL, 100μg/mL） for inhibitory action about anchoring dependence growth, soft agar colony forming ability and nuceic acid synthesis of T-47D cells. Results： （1） With concentration of Hedyotic diffusa alcohol extractive heightening, growth of T-47D cells was obviously inhibited （2） Increasing density of Hedyotic diffusa alcohol extractive was able to inhibit nucleic acid synthesis of exponential phase of growth in T-47D cells. Conclusion： Hedyotic diffusa alcohol extractive affected nucleic acid synthesis of T-47D cells to inhibit cellular growth. |
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| Keywords: | Hedyotic diffusa Breast cancer cell line T-47D Growth inhibiting |
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