Human heat shock gene expression and the modulation of plasma membrane Na+, K+-ATPase activity |
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Authors: | R H Burdon C M Cutmore |
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Institution: | Departments of Pathology and Biochemistry, University of Missouri School of Medicine, Columbia, MO 65212, USA |
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Abstract: | Benzodiazepine receptor solubilized from bovine cortical membranes was bound to a new benzodiazepine affinity column, the synthesis of which is described. Bio-specific elution with the benzodiazepine compound chlorazepate resulted in the elution of fractions highly enriched in specific binding for the GABA receptor agonist muscimol. Specific activity for 3H]muscimol binding was >1.3 nmol/mg protein. It is shown that 3H]flunitrazepam binding activity can be recovered by removal of chlorazepate from the purified fraction. These results strongly support a model which suggests that the 2 binding sites reside on the same physical entity. |
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Keywords: | inorganic phosphate TFP trifluoperazine 3 3′-dipropylthiodicarbocyanine iodide CDR calcium-dependent regulatory protein |
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