| 雌雄异株葡萄的性别鉴定研究 |
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| 引用本文： | 张立平,林伯年,沈德绪.雌雄异株葡萄的性别鉴定研究[J].植物学报,1998,15(4):63-67. |
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| 作者姓名： | 张立平 林伯年 沈德绪 |
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| 作者单位： | 1(浙江农业大学生物科学系 杭州 310029） 2（浙江农业大学园艺系 杭州 310029） |
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| 摘 要： | 通过扫描电镜观察花粉的形态；分析叶片超氧化物歧化酶同工酶(SOD)；RAPD分析基因组DNA多态性，来鉴别葡萄的性别。结果表明：葡萄雌雄株的花粉形态存在着较大的差异；在本实验条件下葡萄雌雄株叶片SOD同工酶谱带一致；一些引物的RAPD产物间存在着多态性，在葡萄雌雄株的基因组DNA序列中存在着碱基排列顺序的差异。实验指出，花粉形态和基因组DNA的多态性分析是进行性别鉴定的有效方法。
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| 关 键 词： | 葡萄 雌雄异株 性别鉴定 |
The Study on Sexual Distinction of Dioecism in Vitis |
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| ZHANG Li-Ping,LIN Bo-Nian and SHEN De-Xu.The Study on Sexual Distinction of Dioecism in Vitis[J].Bulletin of Botany,1998,15(4):63-67. |
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| Authors: | ZHANG Li-Ping LIN Bo-Nian and SHEN De-Xu |
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| Institution: | 1(Department of Biology Science, Zhejiang Agricultural University, Hangzhou 310029) 2(Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029) |
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| Abstract: | We differentiated the sexual distinction of dioecism Vitis by observing the shape of pollen under a scanning electron microscope; analyzing the isozyme of SOD in leaves; detecting the polymorphism of genomie DNA with the method of RAPD. The resulsts indicated that:there was a great difference in the pollen shape of male and female Vitis, the pattern of SOD isozyme were the same in male and female dioecism Vitis; some of the results of RAPD were polymorphic between the male and female. The shape of pollen and RAPD were effective for differentiating the sexual distinction of dioecism. |
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| Keywords: | , |
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