An Inexpensive Microphotometer System for Measuring Silver Grain Densities in Autoradiographs |
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| Authors: | Howard C. Hughes Patricia M. Meyer James W. Meyer Donald R. Meyer Jacqueline C. Bresnahan |
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| Affiliation: | a Laboratory of Comparataive and Physiological Psychology, and Department of Anatomy, The Ohio State University, Columbus, Ohiob Energy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts |
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| Abstract: | An inexpensive microphotometric system is described which will facilitate reading grain-dense areas in autoradiographic material. It is reliable, easy to operate and adaptable to most light microscopes with a dark field condenser. in addition to the microscope, the components include a photodiode, an operational amplifier and a digital mV meter. Data are presented to demonstrate the sensitivity of the system and to illustrate the differential variability between the microphotometer ad the counting of grains by eye. |
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