Identification of biotypes and secondary endosymbionts of Bemisia tabaci in Korea and relationships with the occurrence of TYLCV disease |
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Authors: | Jungan Park SM Hemayet Jahan Woo-Geun Song Hyejung Lee Young-Su Lee Hong-Soo Choi Kwan-Suk Lee Chang-Suk Kim Sukchan Lee Kyeong-Yeoll Lee |
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Institution: | 1. Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea;2. School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea;3. Environmental Agriculture Research Division, Gyeonggi-do Agricultural Research and Extension Services, Hwasung 445-784, Republic of Korea;4. National Institutes of Agricultural Science, Suwon 441-707, Republic of Korea |
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Abstract: | Bemisia tabaci is a species complex that consists of at least 24 genetically diverse biotypes. Here, we determined the biotypes of 27 populations collected in 17 different regions of Korea. Nucleotide sequence comparisons of cytochrome oxidase showed that 26 populations were Q biotype and that one population, the Goyang population, was B biotype. Further subgroup analysis of the Q biotype showed that all populations belonged to the Q1 subgroup, which originates from Western Mediterranean countries. Five endosymbiotic bacteria from various B. tabaci populations were analyzed by comparing rDNA sequences. Hamiltonella was detected in all the populations tested regardless of biotype. Cardinium was detected in all Q biotype populations but not in the B biotype population, while Rickettsia was detected in the B biotype population but not in Q biotype populations. Arsenophonus and Wolbachia were detected in 35% and 58% of Q biotype populations, respectively, but not in the B biotype population. Our results show that the endosymbiont profile is strongly associated with each biotype and with subgroups of the Q biotype. Survey of TYLCV disease from 2008 to 2010 indicated that this disease is widely spread in Korea. This study suggests that the rapid spread of TYLCV may be associated with endosymbiont infection, particularly Hamiltonella infection of B. tabaci. |
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