Non-tuberculous mycobacteria and their surface lipids efficiently induced IL-17 production in human T cells |
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| Authors: | Bodil Jönsson Malin Ridell Agnes E Wold |
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| Institution: | 1. Clinical Bacteriology Section, Department of Infectious Medicine, Institute of Biomedicine, University of Gothenburg, Guldhedgatan 10A, Göteborg SE-413 46, Sweden;2. Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Göteborg, Sweden;1. Department of Information Engineering, University of Parma, Parma, Italy;2. Department of Veterinary Sciences, University of Torino, Italy;3. Molecular Biotechnology Center, University of Torino, Italy;1. Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine Detroit, MI 48201, United States;2. Department of Pathology, Case Western Reserve University Cleveland, OH 44106, United States;3. Division of Internal Medicine, Harbor Hospital Baltimore, MD 21225, United States;4. Experimental Retrovirology Section, HIV and AIDS Malignancy Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, United States;5. Life Sciences Collaborative Access Team and Department of Molecular Pharmacology and Biological Chemistry Northwestern University Feinberg School of Medicine Chicago, IL 60611, United States;1. Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States;2. Angiogenesis and Regenerative Medicine Sector, Dr. H. Afsar Lajevardi Research Cluster, Shiraz, Iran;3. Department of Pediatircs, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States;4. Department of Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States;5. Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States;1. Department of Neurology, Justus-Liebig-University Giessen, Klinikstrasse 33, 35392 Giessen, Germany;2. Heart & Brain Research Group, Justus-Liebig-University Giessen and Kerckhoff Clinic, Benekestrasse 2-8, 61231 Bad Nauheim, Germany;3. Department of Neuroradiology, Justus-Liebig-University Giessen, Klinikstrasse 33, 35392 Giessen, Germany;4. Max-Planck-Institute for Heart and Lung Research, Ludwigstraße 43, 61231 Bad Nauheim, Germany;5. Department of Neurology, Buergerhospital Friedberg, Ockstaedter Strasse 3-5, 61169 Friedberg, Germany |
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| Abstract: | Interleukin-17 (IL-17) is produced by a subset of CD4+ T helper (Th) lymphocytes known as Th17 cells. In humans, IL-1β, enhanced by IL-6 and IL-23 is crucial for differentiation of these cells. IL-17 evokes inflammation and is involved in host defence against microorganisms, although little is known about its role in diseases caused by non-tuberculous mycobacteria. The genus Mycobacterium contains both obligate and opportunistic pathogens as well as saprophytes, and the mycobacterial cell envelope is unique in its abundance of lipids. Here we investigated IL-17 and IL-23 production in human PBMC in response to intact UV-inactivated mycobacteria and mycobacterial surface lipids from two opportunistic (Mycobacterium avium and Mycobacterium abscessus) and one generally non-pathogenic (Mycobacterium gordonae) species. Representative Gram-positive (Enterococcus faecalis, Streptococcus mitis) and Gram-negative (Escherichia coli) bacteria were included as controls. Intact mycobacteria induced production of large amounts of IL-17, while IL-17 responses to control bacteria were negligible. Purified CD4+ T cells, but not CD4-depleted cell fractions, produced this IL-17. Isolated mycobacterial surface lipids induced IL-17, but not IL-23 production. The ability of the non-tuberculous mycobacteria to induce IL-17 production in CD4+ T cells was the same regardless of the pathogenic potential of the particular mycobacterial species. |
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