Metabolic effects of a bacterial lysine decarboxylase gene expressed in a hairy root culture ofNicotiana glauca |
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| Authors: | L F Fecker S Hillebrandt C Rügenhagen S Herminghaus J Landsmann J Berlin |
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| Institution: | (1) Biologische Bundesanstalt f. Land- und Forstwirtschaft, D-3300 Braunschweig, Germany;(2) GBF-Gesellschaft f. Biotechnologische Forschung m.b.H., Mascheroder Weg 1, D-3300 Braunschweig, Germany |
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| Abstract: | OneNicotiana glauca line with distinctly enhanced levels of lysine decarboxylase (LDC) activity and of cadaverine was detected among 54 hairy root cultures of different tobacco species, transformed with the binary vector pLX222 carrying a bacteial lysine decarboxylase gene directed by the 35S-promoter of CaMV. Anabasine levels of this line were nearly doubled in comparison to control lines transformed with the gus-gene instead of the ldc-gene. |
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